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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Dev Biol. 2009 Dec 16;338(2):270–279. doi: 10.1016/j.ydbio.2009.12.008

Figure 5.

Figure 5

Agr2 knockout increases ER stress in the intestine. (A) Germline Agr2−/− small intestine has increased levels of the ER stress response marker BiP. Agr2 protein and actin loading control are shown below. (B) Inducible Agr2−/− small intestine (day6) has increased ER stress response marker Xbp1s. Agr2 deletion was induced with tamoxifen and small intestine was assayed for Xbp1s mRNA on day6. Xbp1u, unspliced, full length form of Xbp1. Xbp1s, spliced form that is induced by ER stress. (C) Increased ER stress marker mRNA expression in induced Agr2−/− mice (day 6). qPCR of ER stress biomarker expression levels of Chop, Pdia3 and Perk. mRNA expressions of wild type are normalized to 1.0. Mean bars of standard error are shown. The indicated P-values were determined by Student t-test.