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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Int J Cancer. 2011 Jan 1;128(1):132–143. doi: 10.1002/ijc.25330

Figure 3.

Figure 3

The role of miR-16-2 in esophageal cancer cells. A, Northern blotting. Esophageal adenocarcinoma SKGT-4 cells and squamous cell carcinoma TE-3 cells were grown and treated with 1 μl BPDE for 12 h and RNA was isolated and subjected to northern blot analysis. B, SKGT-4 and TE-3 cells were grown in monolayer and transiently transfected with pRNA-u6.3/miR-16-2 vector or vector-only control and treated with G418 for 3 days. RNA from the cells was isolated and subjected to RT-PCR analysis of RAR-β2 expression. C, Esophageal adenocarcinoma SKGT-5 cells and squamous cell carcinoma HEC-7 cells were grown and transiently transfected with pRNA-u6.3/miR-16-2 vector or vector-only control and treated with G418 for 4 days. The cells were then subjected to cell viability assay.