Skip to main content
. 2001 Feb 13;98(4):1999–2004. doi: 10.1073/pnas.98.4.1999

Figure 1.

Figure 1

Induction of DNA dsb in mIMCD3 cells by HNa. Neutral comet assay was used to measure DNA dsb. (A) Control cells kept in isosmotic medium have only a short comet tail. (B) The comet tail increases after exposure of cells for 1 h to 725 mosmol/kg HNa. (C) Exposure of cells for 1 h to 5 milliunit/ml bleomycin also increases the comet tail. (D) Appearance of apoptotic cells when analyzed by neutral comet assay. Note the highly condensed nucleus and the very high ratio of comet tail length and width to nuclear diameter. (E) Quantification of comet moment (fraction of DNA in the comet tail versus the comet head) in mIMCD3 cells. Data are means ± SEM (n = 3).