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. 2001 Feb 13;98(4):1999–2004. doi: 10.1073/pnas.98.4.1999

Figure 2.

Figure 2

HNa but not HU increases DNA dsb in mIMCD3 cells. PFGE was used to measure DNA dsb. (A) Representative PFGE gel illustrating the rate of migration of whole genomic DNA from cells exposed to isosmotic medium (300 mosmol/kg), 600 mosmol/kg HNa, 600 mosmol/kg HU, and 5 or 50 milliunit/ml bleomycin. (B) Quantification of DNA dsb by PFGE. The fraction of DNA recovered (FAR), which is the amount of DNA that has migrated from the well into the lane vs. total DNA in lane plus well, is plotted. Data shown are means ± SEM (n = 3).