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. 2001 Feb 13;98(4):1999–2004. doi: 10.1073/pnas.98.4.1999

Figure 5.

Figure 5

Solute-specific effects of inhibition of DNA-activated protein kinases on mIMCD3 cells exposed to hyperosmotic stress. (A) Morphology of mIMCD3 cells after growing them for 15 h in various media with or without 25 μM LY294002. (Magnification, ×400.) NaCl/urea 600 = 600 mosmol/kg HNa/HU, NaCl/urea 800 = 800 mosmol/kg HNa/HU. (B and C) Effect of LY294002 on number of cells left in a confluent 10-cm dish after 15 h culture in various osmolalities. (B) HNa. Data are means ± SEM (n = 3). Note that cell number drops significantly after exposure to 600 mosmol/kg with LY294002 but not without it. (C) HU. Data are means ± SEM (n = 3). Unlike with HNa, cell numbers are not significantly different between 300 and 600 mosmol/kg even in the presence of LY294002 during HU. When error bars are not visible, they are smaller than the corresponding symbol.