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. 2001 Feb 13;98(4):1999–2004. doi: 10.1073/pnas.98.4.1999

Figure 6.

Figure 6

Suppression of cell growth and DNA repair by the DNA-activated protein kinase inhibitor LY294002. DNA dsb in mIMCD3 cells exposed to 600 mosmol/kg HNa are significantly increased at 24 h in isosmotic controls and in cells treated with 25 μM LY294002 plus HNa but not in cells treated with 25 μM LY294002 in isosmotic medium. DNA dsb continue to increase significantly when cells are left in HNa plus 25 μM LY294002, whereas they decrease to baseline by day 3 in HNa without LY294002. There is a small but significant increase in DNA dsb at 72 h when cells are exposed to 25 μM LY294002 in isosmotic medium. However, this increase is much smaller compared with the effect of LY294002 in HNa. DNA dsb were measured by PFGE; FAR = amount of DNA in lane/amount of DNA in (lane plus well). Data are means ± SEM (n = 3).