Figure 7.

Monodansylcadaverine-labeled vacuoles in HepG2 cells. Autophagic vacuoles were labeled with 0.05 mmol/L monodansylcadaverine (MDC) in phosphate-buffered saline (PBS) at 37°C for 10 min. The fluorescent density and the MDC-labeled particles in HepG2 cells were higher in matrine treatment group than in control group. The number of MDC-labeled particles in HepG2 cells was significantly lower in combined 3-MA and matrine treatment group than in single matrine treatment group (× 400 magnifications). A: Control; B: Matrine; C: 3-MA; D: 3-MA + matrine.