Fig. 1.

Single-channel recordings of Kir2.1/Kir2.2 channels. a Representative recordings of Kir2 single-channel currents recorded in HEK293 cells at −100 mV in cell-attached mode with high external K⁺. b Examples of recordings that contain small channels (left) and sub-conductance states (right). Left, a single-channel recording from a patch expressing Kir2.2 monomeric channels. A smaller conductance channel can be observed with a larger conductance channel (black triangle). Right, a single-channel recording from a patch expressing Kir2.1-2-1-2 heteromeric channel. Insert, an enlargement of the section of the recording where sub-conductance states are seen