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. Author manuscript; available in PMC: 2010 Sep 12.
Published in final edited form as: Nat Neurosci. 2009 Apr 19;12(5):568–576. doi: 10.1038/nn.2318

Figure 4.

Figure 4

Association with Myosin Va is sufficient for dendritic targeting. (a c) CD8 fused to a Myosin Va binding domain (CD8-MBD) and expressed with GFP in a cortical neuron localized specifically to the soma and dendrites. (c) Camera lucida drawing. Axon, black; somatodendritic region, gray. (d–f) CD8 expressed with GFP localized to both axons and dendrites. (f) Camera lucida drawing. (g) CD8-MBD, which associates with Myosin Va, localized dendritically as assessed by either total protein or surface protein staining (ADRCD8-MBD = 0.13 ± 0.02; ADRCD8-MBD,surface = 0.19 ± 0.04). In contrast, CD8 localized nonspecifically in either case (ADRCD8 = 0.90 ± 0.08; ADRCD8,surface = 0.80 ± 0.13). CD8-MBD localized nonspecifically when expressed with HA-dnMVa (ADRCD8-MBD,dnMVa = 1.14 ± 0.23). Error bars, s.e.m.; arrow, axon; arrowheads, axon initial segment. Insets, staining for Ankyrin G. Scale bars, 10 μm. *P < 0.0002; NS, P > 0.08 (Wilcoxon-Mann-Whitney).