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. 2001 Feb 6;98(4):2005–2010. doi: 10.1073/pnas.041591798

Figure 2.

Figure 2

Effect of complete Acrp30 and globular region of Acrp30 (gAcrp30) on plasma FFA, glucose, and triglyceride levels in C57BL/6J mice after a high-fat meal. (a–c) Two groups of animals (▴, control; ⧫, gAcrp30-treated; n = 8, each) were fasted for 3 h before the experiment before a baseline blood sample was taken. A high-fat/sucrose meal was given by gavage (vol. = 1% of body weight). Saline or 25 μg gAcrp30 was injected immediately after the high-fat meal and again at 45 min and at 1 h 45 min. Treatment with gAcrp30 resulted in a significant reduction of plasma FFAs at 1–4 h and of glucose and triglycerides at 2–4 h (P < 0.05). (d–f) A similar experiment was conducted in three groups of animals (▴, control, n = 6; ⧫, 3 × 25 μg; ●, 3 × 50 μg full-length Acrp30 treated, n = 4 each). In contrast to the globular head protein, treatment with Acrp30 showed only very small effects, and a significant reduction of plasma FFAs was only seen at 2 h, and a reduction of glucose was seen at 3 h (P < 0.05). All control animals were injected with saline. Blood samples were immediately put on ice; plasma was prepared and kept at −20°C; and triglycerides, FFAs, and glucose were determined within 24 h.