Figure 1.
Effect of an adenovirus vector with no transgene (AdNull) on endogenous human immunodeficiency virus (HIV)–1 replication by human alveolar macrophages (AMs). AMs were isolated from an HIV-1+ individual not on treatment with highly active antiretroviral therapy (HAART). The AMs (2 × 105/well) were infected with AdNull 2.5 × 104 particle units (pu)/cell, treated daily with 10 μg/ml 3′-azido-3′-deoxythymidine (AZT), or left untreated. Aliquots of media were collected every 4 days and assayed for HIV-1 p24 protein to quantify viral replication. Each symbol represents the measurement from a single well. Ordinate, p24 (ng/ml); abscissa, time (d). The results were similar in AMs obtained from five different individuals.