Figure 2.

Dose- and time-dependent effect of AdNull on endogenous and laboratory-infected HIV-1 replication by human AMs. (A) Time dependency of HIV-1 replication in naive and AdNull-treated AMs. AMs were isolated from an HIV-1⁺ individual not on treatment with HAART. The AMs (2 × 10⁵/well) were infected with AdNull 10² - 2.5 × 10⁴ pu/cell or treated daily with AZT, 10 μg/ml or left untreated. Aliquots of media were collected and assayed for HIV-1 p24 protein to quantify viral replication. Each symbol represents the measurement from a single well. Ordinate, p24 (ng/ml); abscissa, time (d). The results were similar in AMs obtained from five different individuals. (B) Effect of time after HIV-1 infection on HIV-1 replication with laboratory infection of AMs with HIV-1. AMs were isolated from a normal volunteer. The AMs (2 × 10⁵/well) were infected with 10² to 2.5 × 10⁴ pu/cell AdNull, treated daily with 10 μg/ml AZT, or left untreated. After 2 hours, all of the wells were washed and infected with an HIV-1 strain isolated from the frontal lobe of subject JR who died of AIDS demetia (JRFL) 10³ median tissue culture infectious dose (TCID₅₀)/well. The media were replaced at Day 12. Aliquots of media were collected at Days 12, 16, and 20, and assayed for HIV-1 p24 protein to quantify viral replication. Ordinate, p24 (ng/ml); abscissa, time (d). The results were similar in AMs obtained from eight different individuals.