Figure 3.
Effect of timing of Ad infection on HIV-1 replication by human AMs infected in vitro with HIV-1. AMs were isolated from a normal volunteer. The AMs (2 × 105/well) were infected with 102 to 2.5 × 104 pu/cell AdNull at Days −3 (before HIV-1 infection), 0, 7, or 14 after HIV-1 infection, treated daily 10 μg/ml with AZT, or left untreated. (A) Ad infection 3 days before HIV-1 infection. (B) Ad infection 2 hours before HIV-1 infection. (C) Ad infection 7 days after HIV-1 infection. (D) Ad infection 14 days after HIV-1 infection. Ordinate, p24 (pg/ml); abscissa, time (d). The results were similar in AMs obtained from three different individuals.