Figure 4.

Effect of Ad on HIV-1 replication by human AMs under conditions where reverse transcription is blocked. AMs were isolated from a normal volunteer. The AMs (2 × 10⁵/well) were infected with HIV-1 JRFL 10³ TCID₅₀/well. At Day 12, the cells were treated with 10 μg/ml AZT. After an additional 4 days, the cells were infected with 2.5 × 10⁴ pu/cell AdNull or left uninfected. AZT was added daily. Aliquots of media were collected and assayed for p24 protein, but the media were not changed until 14 days later, as indicated. Ordinate, p24 (pg/ml); abscissa, time (d). The results were similar in AMs obtained from three different individuals.