Figure 5.
Effect of Ad on the transcription of HIV-1–specific DNA in AMs. To assess the effect of Ad on reverse transcription of HIV-1 RNA to DNA, AMs were infected with 2.5 × 104 pu/cell AdNull, treated with AZT, or left untreated. After 72 hours, the AMs were infected with HIV-1 JRFL, 103 TCID50/well. DNA was then isolated at 0, 24, 48, and 120 hours, and HIV-1 DNA was quantified by TaqMan real-time PCR. Ordinate, HIV-1 DNA copies/μl; abscissa, time (d). The results were similar in AMs obtained from three different individuals.