Figure 6.

Effect of Ad on integrated HIV-1–specific DNA in AMs. To assess the effect of Ad on integration of HIV-1 DNA into the chromosomal DNA, AMs were infected with 2.5 × 10⁴ pu/cell AdNull, treated with AZT, or left untreated. After 1 hour, the AMs were infected with HIV-1 JRFL, 10³ TCID₅₀/well. DNA was isolated at 72 hours and integrated HIV-1 DNA was quantified by a two-step nested TaqMan real-time PCR. The initial PCR step specifically amplifies only sequences annealing to both human 300–base pair repetitive DNA sequences (Alu) primers and a JRFL env primer. In the second step, JRFL primers with an internal probe site quantify the Alu-JRFL amplification products with the first-round PCR products as a template. Ordinate, integrated HIV-1 DNA (copies/μl); abscissa, infection conditions (no virus, JRFL, JRFL + Ad, JRFL + AZT). The results were similar in AMs obtained from three different individuals.