. Author manuscript; available in PMC: 2010 Sep 13.

Published in final edited form as: Nat Rev Mol Cell Biol. 2009 Aug 12;10(9):644–650. doi: 10.1038/nrm2746

Table 1.

Summary of recent progress in cellular reconstitution

Reconstituted process or structure	Components used	References
*Microspheres*
Cell motility	Listeria monocytogenes and NPF-coated microspheres immersed in Xenopus laevis extracts	63,64
	NPF-coated microspheres, actin, profilin, cofilin, Arp2/3 complex and capping protein	22,23
	NPF-coated microspheres, actin, Arp2/3 complex and fascin	25,24
Mitotic spindle	DNA-coated microspheres and X. laevis cytoplasmic extracts	20,65
Plasmid segregation	ParC coated microspheres, ParR and ParM	27
*Supported bilayers*
Immunological synapse	Supported bilayer containing MHC peptide and ICAM1, and T cells	31,62
Neuronal synapse	Supported bilayer containing neuroligin 1, and neuronal cells	32
Bacterial cell division	MinD and MinE	33
Vesicle fission	Dynamin 1 and GTP	35
Cell motility	Lipid-coated beads, N-WASP, actin, profilin, cofilin, Arp2/3 complex and capping protein	34
*Giant vesicles*
Bacterial cell division	Membrane-targeted FtsZ	50
Membrane tube networks	Kinesin-bound GUV and microtubule tracks	40,66
Actin–membrane interaction	PtdIns(4,5)P₂-containing vesicles, actin, N-WASP and Arp2/3 complex	46
Filopodia formation	PtdIns(4,5)P₂-containing vesicles, actin, N-WASP and Arp2/3 complex	48
Membrane scission	vps20, snf7 and vps24	67

Arp2/3, actin-related protein 2/3; GUV, giant unilamellar vesicle; ICAM1, intercellular adhesion molecule 1; MHC, major histocompatibility complex; NPF, nucleation-promotion factor; N-WASP, neuronal-Wiscott–Aldrich syndrome protein (also known as WASL); PtdIns(4,5)P₂, phosphatidylinositol-4,5-bisphosphate; snf7, sucrose non-fermenting protein 7; Vps, vacuole protein sorting protein.