Table 2.
Test materials |
Faecal characteristics |
||||||||
---|---|---|---|---|---|---|---|---|---|
Dose (mg/kg) | % respondent | Latent period (min) | Total stool frequency | Purging indices | % inhibition of defecation | Frequency of wet stool | Water content (g) | Inhibition of wet stool (%) | |
Saline control | 0 | 100 | 60.80 ± 6.62 | 10.20 ± 1.31 | 16.77 | 0 | 7.90 ± 0.73 | 3.22 ± 0.55 0 | 0 |
Methanol extract# | 100 | 100 | 78.60 ± 5.75* | 6.50 ± 2.12* | 8.26 | 36.27 | 3.50 ± 0.70* | 2.05 ± 0.59* | 55.69 |
200 | 100 | 119.9 ± 6.90* | 4.10 ± 0.56* | 3.41 | 59.80 | 2.40 ± 0.84* | 1.47 ± 0.21* | 69.62 | |
400 | 80 | 270.50 ± 37.88* | 3.25 ± 0.67* | 0.96 | 68.13 | 1.50 ± 0.53* | 1.07 ± 0.48* | 81.01 | |
n-hexane fraction | 80 | 100 | 70.00 ± 5.35 | 8.10 ± 1.19* | 11.57 20.58 | 5.00 ± 0.66* | 2.88 ± 0.25* | 36.70 | |
Ethyl acetate fraction | 80 | 60 | 186.50 ± 23.61* | 3.70 ± 0.94* | 1.19 | 63.72 | 2.16 ± 0.98* | 1.03 ± 0.36* | 72.65 |
n-butanol fraction | 80 | 70 | 112.14 ± 15.68* | 5.90 ± 1.59* | 3.68 | 42.15 | 2.71 ± 1.11* | 2.00 ± 0.60* | 65.69 |
Aqueous residue | 80 | 90 | 111.17 ± 19.22* | 5.20 ± 1.03* | 4.20 | 49.01 | 2.66 ± 0.86* | 1.53 ± 0.37* | 66.32 |
Diphenoxylate HCl | 2.5 | 50 | 361.00 ± 29.66* | 2.90 ± 0.70* | 0.40 | 71.56 | 1.20 ± 0.44* | 0.68 ± 0.20* | 84.81 |
Loperamide | 2.5 | 60 | 337.16 ± 26.53* | 3.70 ± 0.67* | 0.65 | 63.72 | 2.16 ± 0.75* | 1.05 ± 0.25* | 72.65 |
Isoliquiritigenina | 2.5 | 50 | 179.40 ± 11.65* | 3.80 ± 0.90* | 1.05 | 62.74 | 1.20 ± 0.44* | 0.88 ± 0.22* | 84.81 |
Values are means ± SD; n = 10 in each group.,
Significantly different from the saline control (P ≤ 0.001) Duncan test;
The methanol extract of T. madagascariens was fractionated into the n-hexane, ethyl acetate, n-butanol and aqueous residues fractions; aIsoliquiritigenin, the isolated compound, was tested at the same dose as the reference drugs (diphenoxylate HCl and loperamide).
Isoliquiritigenin, the isolated compound, was tested at the same dose as the reference drugs (diphenoxylate HCl and loperamide).