Table 4.

Effect of test substances on intestinal charcoal transit in rats

Test substances	Dose (mg/kg)	Intestinal charcoal transit (%)	% inhibition of charcoal transit
Saline control	0	73.07 ± 2.13	0
Atropine sulfate	2.5	31.06 ± 3.69*	57.49
Isoliquiritigenin	2.5	41.39 ± 5.54*	43.35
n-hexane fraction	80	56.33 ± 4.03*	22.90
Ethyl acetate fraction	80	39.33 ± 2.06*	46.17
n-butanol fraction	80	41.48 ± 2.89*	43.23
Aqueous residue fraction	80	54.33 ± 3.93*	25.64
Methanol extract	100	68.01 ± 1.77*	6.92
	200	59.94 ± 2.04*	17.96
	400	53.10 ± 3.23*	27.32

Values are means ± SD; n = 8 in each group;

^*Significantly different from the saline control (P ≤ 0.001) Duncan test;

^#The methanol extract of T. madagascariens was fractionated into the n-hexane, ethyl acetate, n-butanol and aqueous residues fractions;

^aIsoliquiritigenin, the isolated compound, was tested at the same dose as the reference drugs (diphenoxylate HCl and loperamide).