Table 4.
Test substances | Dose (mg/kg) | Intestinal charcoal transit (%) | % inhibition of charcoal transit |
---|---|---|---|
Saline control | 0 | 73.07 ± 2.13 | 0 |
Atropine sulfate | 2.5 | 31.06 ± 3.69* | 57.49 |
Isoliquiritigenin | 2.5 | 41.39 ± 5.54* | 43.35 |
n-hexane fraction | 80 | 56.33 ± 4.03* | 22.90 |
Ethyl acetate fraction | 80 | 39.33 ± 2.06* | 46.17 |
n-butanol fraction | 80 | 41.48 ± 2.89* | 43.23 |
Aqueous residue fraction | 80 | 54.33 ± 3.93* | 25.64 |
Methanol extract | 100 | 68.01 ± 1.77* | 6.92 |
200 | 59.94 ± 2.04* | 17.96 | |
400 | 53.10 ± 3.23* | 27.32 |
Values are means ± SD; n = 8 in each group;
Significantly different from the saline control (P ≤ 0.001) Duncan test;
The methanol extract of T. madagascariens was fractionated into the n-hexane, ethyl acetate, n-butanol and aqueous residues fractions;
Isoliquiritigenin, the isolated compound, was tested at the same dose as the reference drugs (diphenoxylate HCl and loperamide).