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The virulence of the tet-RFG1 strain is unaffected by the presence or absence of doxycycline. Approximately 3.5 × 105 C. albicans yeast cells of the tet-RFG1 strain PMC-X7 were injected into mice that had been placed on either 5% sucrose (No DOX) or 5% sucrose containing 2 mg/ml of doxycycline (Plus DOX). There was no difference in the survival curves produced under the two conditions.
