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5′ RACE analysis reveals two 5′ termini for the UPC2 transcript. RNA from SC5314 was prepared and used as the template in 5′ RACE analysis. Resulting PCR products were analyzed using gel electrophoresis on a 1.5% agarose gel. Bands were extracted from the gel, and 5′ termini were determined by DNA sequencing. Lanes are from two sections of the same gel. Bands are labeled for the position within the gene (bp −361 and −148) and not the size of the gel fragment.
