Fig. 3.

Azole inducibility of 100-bp nested deletions of the UPC2-RLUC reporter in the WT and the UPC2 homozygous deletion strain. Deletion constructs of 100 bp are listed on the x axis, with the length of the construct representing the distance upstream of the ATG codon. Luciferase assays were performed after 48 h of growth in the presence and absence of 100 μg/ml FLC. Data are presented as the proportion of azole induction of RLUC activity of each deletion construct relative to the activity in the full-length promoter construct. Wild-type BWP17 is shown in black and the upc2Δ/upc2Δ strain in gray.