Fig. 7.

CaUpc2p binds to the SREs within the UPC2 promoter. (A) An electrophoretic mobility shift assay (EMSA) was performed using the purified Upc2p DNA binding domain (rDBD) and Cy5-labeled oligonucleotides containing the SRE from ERG11 (left) or UPC2 (right). Unlabeled competitors include SRE-A and SRE-B as well as mutated versions of SRE-A and SRE-B. Unlabeled competitors were used at 25-fold molar excess. (B) The percent binding or gel shift of the labeled SRE-A probe (y axis) was determined in the presence of increasing amounts of cold competitor of SRE-A (circles) or SRE-B (squares) (x axis).