Fig. 8.

CaUpc2p requires an intact SRE and a second element (SDR) for binding. An EMSA was run with the labeled UPC2 SRE-A probe in the presence of cold competitor as shown in the figure. Black bars represent the area that was mutated in the cold competitor, as described in Materials and Methods. Arrows show that the oligonucleotide sequence continues in that direction, as shown in Table S1 in the supplemental material. Competition was determined compared to the gel shift in the absence of competitor. Oligos, oligonucleotides.