FIG. 7.

Expression of gfp from the pdh promoter in biofilms. Representative images of static biofilms of S. mutans SL15013 established with CDM containing 3 mM sucrose are shown. Cultures were inoculated in 24-well plates containing sterile 12-mm-diameter glass coverslips. The plates were incubated at 37°C in 5% CO₂. At the indicated times, the coverslips were washed with PBS and imaged. An overlay of GFP fluorescence on differential interference contrast images is shown. Bars, 5 μm.