FIG. 4.
Gel electrophoresis of PNPase digests of the 5601 transcript. 32P-labeled 5601 RNA was synthesized as described previously (9). Gels were subjected to autoradiography. Lane 1, undigested 5601 transcript; lanes 2 and 3, digestion with S. antibioticus PNPase; lanes 4 and 5, digestion with S. coelicolor PNPase; lanes 6 and 7, digestion with E. coli PNPase. The reaction mixtures corresponding to lanes 3, 5, and 7 contained 1 mM ppGpp. The product designations at the left and right of the figure relate the bands produced to the 3′-endpoints mapped previously by cDNA cloning and sequencing (9).