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. 2010 Jun 25;192(17):4275–4280. doi: 10.1128/JB.00367-10

TABLE 1.

Kinetic parameters for the polymerization reactions in the presence or absence of ppGppa

ppGpp concn (mM) Mean ± SEM
kcat/Km (l mol−1 min−l, 103)
Km (mM)
kcat (min−1)
S. antibioticus S. coelicolor E. coli S. antibioticus S. coelicolor E. coli S. antibioticus S. coelicolor E. coli
0 1.46 ± 0.12 6.14 ± 0.43 14.6 ± 1.2 80.5 ± 2.9 492 ± 43.4 731 ± 224 55.1 80.1 50.1
0.5 4.24 ± 0.77 4.68 ± 0.32 12.6 ± 2.0 59.1 ± 6.5 141 ± 14.1 672 ± 179 13.9 30.1 53.3
1.0 2.78 ± 0.26 3.96 ± 0.68 - 21.2 ± 4.2 59.3 ± 6.7 - 7.6 15.0
a

The Km and kcat data are averages of two sets of duplicates.