TABLE 1.
ppGpp concn (mM) | Mean ± SEM |
kcat/Km (l mol−1 min−l, 103) |
|||||||
---|---|---|---|---|---|---|---|---|---|
Km (mM) |
kcat (min−1) |
||||||||
S. antibioticus | S. coelicolor | E. coli | S. antibioticus | S. coelicolor | E. coli | S. antibioticus | S. coelicolor | E. coli | |
0 | 1.46 ± 0.12 | 6.14 ± 0.43 | 14.6 ± 1.2 | 80.5 ± 2.9 | 492 ± 43.4 | 731 ± 224 | 55.1 | 80.1 | 50.1 |
0.5 | 4.24 ± 0.77 | 4.68 ± 0.32 | 12.6 ± 2.0 | 59.1 ± 6.5 | 141 ± 14.1 | 672 ± 179 | 13.9 | 30.1 | 53.3 |
1.0 | 2.78 ± 0.26 | 3.96 ± 0.68 | - | 21.2 ± 4.2 | 59.3 ± 6.7 | - | 7.6 | 15.0 |
The Km and kcat data are averages of two sets of duplicates.