TABLE 2.
Rates of germination of B. megaterium GC500 (ΔgerWB) spores complemented with various germinant receptor genesa
| Strain | Genotype | Rate of spore germination with indicated germinantb |
|||||
|---|---|---|---|---|---|---|---|
| Buffer | GPLK | Glucose | Proline | Leucine | KBr | ||
| GC500 | ΔgerWB | <1 | <1 | <1 | <1 | <1 | <1 |
| GC503 | ΔgerWB pHT-gerUA-gerUC | <1 | 4 | <1 | <1 | <1 | 4 |
| GC504 | ΔgerWB pHT-gerUA-gerUC-gerWB | 1 | 100 | 91 | <1 | 85 | 4 |
| GC501 | ΔgerWB pHT-gerUA-gerUC-gerVB | <1 | 100 | 100 | 100 | 43 | 100 |
| GC502 | ΔgerWB pHT-gerUA-gerUC-gerUB | <1 | 100 | 76 | 7 | 60 | 8 |
a
Spores were germinated in 5 mM Tris-HCl, pH 7.8, for 60 min with 10 mM germinant (50 mM KBr). GPLK is a mixture of glucose, proline, and leucine (each at 10 mM) and KBr (50 mM). Spore germination was measured as described in Materials and Methods.
b
Rates of spore germination are given relative to the OD600 loss (65%) for spores of strain QM B1551 in 10 mM glucose, which was set equal to 100. This value is equivalent to 100% germination after 60 min incubation. Values are the means of at least duplicate experiments; the standard deviation was ≤5% of the mean.