TABLE 3.

Rates of germination of spores with GerVB with substitutions at predicted transmembrane-located charged and proline residues^a

Strain	Domain	Substitution	Rate of spore germination with indicated germinantb
			Buffer	GPLK	Glucose	Proline	Leucine	KBr
GC501		None	1	100	99	100	57	100
GC505	TM1	E19Q	<1	100	94	96	51	92
GC506	TM3	D93N	<1	90	68	53	4	62
GC507	TM4	E112Q	3	97	82	87	8	67
GC508	TM4	H119N	<1	99	78	80	1	7
GC509	TM6	E196Q	<1	96	6	1	21	74
GC510	TM6	E196D	<1	97	21	<1	<1	5
GC511	TM8	K282A	<1	94	81	87	25	79
GC512	TM10	H341N	<1	98	82	82	40	69
Strains with proline substitutions
GC513	TM1	P27A	<1	99	69	77	<1	3
GC514	TM4	P114A	1	59	29	34	5	<1
GC515	TM6	P193A	4	88	15	30	45	80
GC516	TM9	P309A	<1	100	86	93	13	86
GC517	TM10	P343A	<1	88	3	<1	<1	6
GC518	TM10	P349A	<1	79	58	64	34	60

^aSpores were germinated in 5 mM Tris-HCl, pH 7.8, for 60 min with 10 mM germinant (50 mM KBr). GPLK is a mixture of glucose, proline, and leucine (each at 10 mM) and KBr (50 mM). Spore germination was measured as described in Materials and Methods.

^bRates of spore germination are given relative to the OD₆₀₀ loss (65%) for spores of strain QM B1551 in 10 mM glucose, which was set equal to 100. This value is equivalent to 100% germination after 60 min incubation. Values are the means of three independent experiments conducted with the same spore preparations; the standard deviation was ≤5% of the mean. Similar values were obtained with other spore preparations.