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Genotyping analysis carried out with single knockout mutants for plasmid-located effector genes hopW1-1 and hopW1-2. PCR analyses were carried out using primers to amplify each of the two genes plus approximately 600 bp of their 5′ and 3′ flanking regions. Genomic DNA from either Pph1448a or its ΔhopW1-1 and ΔhopW1-2 mutant derivatives was used as templates. Arrows indicate the bands corresponding to the wild-type (hopW1-1/hopW1-2) or mutant (ΔhopW1-1::nptII/ΔhopW1-2::nptII) alleles.
