FIG. 7.

(A) Disease symptoms caused by PtoDC3000 expressing Pph1448a effectors in Arabidopsis thaliana. Plants were sprayed with a bacterial suspension of PtoDC3000 or Pto expressing different effectors from the Pph1448a secretome, containing 5 × 10⁷ CFU/ml in 10 mM MgCl₂ and 0.02% Silwet L-77. After inoculation, plants were covered to keep humidity high, and symptoms were documented by 7 dpi. (B) Competitive index assays of PtoDC3000 bacteria coinoculated into either Arabidopsis Col-0 or NahG plants, together with DC3000 strains expressing different effector genes. Four- to five-week-old Arabidopsis plants were inoculated with a 5 × 10⁴-CFU/ml mixed bacterial suspension, containing equal numbers of CFU of wild-type and effector-expressing bacteria, and leaves were sampled at 7 dpi. Competitive indices correspond to the means for three samples. Error bars represent the standard errors. Asterisks indicate results significantly different from 1, as established by Student's t test (P < 0.05).