TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or description | Source, reference, or construction |
|---|---|---|
| Strains | ||
| BW27784 | Δ(araBAD)567 Δ(rhaBAD)568 | Yale E. coli Genetic Stock Center; 18 |
| Δ(araFGH) Φ(ΔaraEpPCP18-araE) | ||
| JW1850-2 | ruvA786(del)::kan; Keio collection | Yale E. coli Genetic Stock Center; 2 |
| JW3627-1 | recG756(del)::kan; Keio collection | Yale E. coli Genetic Stock Center; 2 |
| JW1852-1 | ruvC789(del)::kan; Keio collection | Yale E. coli Genetic Stock Center; 2 |
| JHS4 | BW27784ruvA786(del)::kan | P1(JW1850-2) × BW27784, Kanr |
| JHS5 | BW27784 recG756(del)::kan | P1(JW3627-1) × BW27784, Kanr |
| JHS7 | BW27784ruvC789(del)::kan | P1(JW1852-1) × BW27784, Kanr |
| JJC671 | AB1157ruvA60::Tn10 (ruvB polar) [pGB2-RuvAB] | Bénédicte Michel; 23 |
| JHS6 | BW27784 ruvA60::Tn10 (ruvB polar) | P1(JJC671) × BW27784, Tetr |
| Plasmids | ||
| pETOP | With E. coli topA under control of BAD promoter, high copy number | 4 |
| pETOP-G116S | pETOP with G116S mutation | Site-directed mutagenesis of pETOP |
| pAYTOP | With Y. pestis topA under control of BAD promoter, medium copy number | 5 |
| pAYTOP-G122S | pAYTOP with G122S mutation | 34 |
| pDinlux | SOS reporter plasmid with dinD1::luxCADBE fusion | 5 |
| pGB2 | Cloning vector | Bénédicte Michel; 6 |
| pGB2-RuvAB | Contains entire ruvAB operon | Bénédicte Michel; 32 |
| pGB2-RuvA+ RuvB-Y184H | RuvAB proficient in recombination but not RFR | Bénédicte Michel; 20 |