TABLE 2.
Plasmids used in this study
| Plasmid | Relevant feature(s) | Source or reference |
|---|---|---|
| pKD4 | Template plasmid for gene disruption | 9 |
| pTrc99a | Expression vector; AprPtrc rrnB TtT2ori(pBR322) lacIq | 1 |
| pCP20 | FLP-recombination plasmid: flp bla cat rep101(Ts) | 6 |
| gXRC | Destination vector-cloned GW cassette and mCherry into pXGFP4 | 36 |
| gXGC | Same as gXRC, except mCherry is replaced by GFP | This study |
| pAM1 | rfp fusion vector (pTrc99a-Gateway cassette-mCherry) | This study |
| pAM2 | Ptrc::murG-mCherry bla | This study |
| pAM4 | Ptrc::murG(E268A)-mCherry bla | This study |
| pAM7 | Ptrc::otsA-mCherry bla | This study |
| pAM8 | Ptrc::murG(L79E F82E)-mCherry bla | This study |
| pAM9 | Ptrc::murG-mCherry otsA-GFP bla | This study |
| pAM10 | Ptrc::murG-mCherry ibpA-GFP bla | This study |
| pAM11 | Ptrc::murG-mCherry mraY bla | This study |