TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics or use | Reference or source |
|---|---|---|
| Corynebacterium strains | ||
| C7 (−) | C. diphtheriae wild type | 3 |
| 1737 | C. diphtheriae wild type, Gravis biotype | 29 |
| C7chrSΔ | chrS deletion mutant of C7 (−) | 4 |
| C7ChrSAΔ | chrSA deletion mutant of C7 (−) | 5 |
| C7hrrSAΔ | hrrAS deletion mutant of C7 (−) | 5 |
| C7hrtABΔ | hrtAB deletion mutant of C7 (−) | This study |
| C7dtxRΔ | dtxR deletion mutant of C7 (−) | This study |
| CU44 | C. ulcerans hmuU mutant | 31 |
| E. coli strains | ||
| DH5α | Cloning strain | Invitrogen |
| S17-1 | RP4 mobilization functions | 47 |
| TOP10 | Cloning of PCR fragments | Invitrogen |
| XL1-Gold | QuikChange cloning | Stratagene |
| Plasmids | ||
| pCR-Blunt-II | Cloning of PCR fragments, Kanr | Invitrogen |
| pCM502 | Promoter probe vector, Cmr | 32 |
| pSPZ | Promoter probe vector, Spcr | 24 |
| phrtB-lacZ | hrtB promoter-lacZ fusion on pSPZ | This study |
| pCMtox | tox promoter-lacZ fusion on pCM502 | 32 |
| pKN2.6 | C. diphtheriae shuttle vector, Knr | 37 |
| pKN2.6Z | C. diphtheriae shuttle vector, Knr | 13 |
| pCM2.6 | C. diphtheriae shuttle vector, Cmr | 37 |
| pECK18mob2 | Corynebacterium cloning vector, Knr | 30 |
| pECK-chrSA | chrSA genes cloned into pECK18mob2 | 5 |
| pTrc99A | E. coli cloning and expression vector, Ampr | Pharmacia |
| pKN-chrS | chrS cloned into pKN2.6 | This study |
| pKN-hrtAB | hrtAB cloned into pKN2.6Z | This study |
| pMnt-hrtAB | Plasmid pKN-hrtAB with mntA promoter | This study |
| p2.6-dtxR | dtxR gene cloned into pKN2.6Z | This study |