TABLE 2.
Primers used in this study
| Primer | Amplified region | Sequencea (5′-3′) |
|---|---|---|
| Deletion mutants | ||
| 23/24KO-Ds-F | Downstream of hrtAB | GCATTCTAGATCATCACATTCAGCAATGC |
| 23/24KO-Ds-R | TAGCGTCGACATGGCCGACGGAAAAGCC | |
| 23/24KO-Us-F | Upstream of hrtAB | GACTGTCGACACGAATGTCGCGGATTCC |
| 23/24KO-Us-F | GACTGTCGACACGAATGTCGCGGATTCC | |
| dtxR-UP1 | Upstream of dtxR | GGCAGTACTTCAATGCTGAAGATGAGG |
| dtxR-UP2 | GGCGTCGACGATCCTAGCGCGAAGAGG | |
| dtxR-DN1 | Downstream of dtxR | GGCGTCGACGCCCACACTATTCGTATC |
| dtxR-DN2 | GGCACTAGTAGTACTGACCGAATATCCTTCACC | |
| hrtB promoter fusion | ||
| DIP2324-PO-R | hrtB | GGATCCGGCCGGTCAGCATAACG |
| DIP2324-PO-F | Promoter | GTCGACGGGTTCCTACGCTGACC −3′ |
| hrtAB cloning | ||
| DIP2323-R | CTGAGGATCCGACTAGCACACCAAATTCC | |
| DIP2324-PO-F(RV) | GGCGATATCGGGTTCCTACGCTGACC | |
| 2324P | GGCGATATCCCTTACTTTCACAACAAT | |
| mntP-Rev | GGCGATATCTCCTGTTTGACAAACTTG | |
| chrS cloning | ||
| chrS-kin-R | GTCGACGGATCATGAAGTCTCCCG | |
| chrS-wt-F | GGATCCGCAGGAGATCTTTTATGC | |
| ChrS C-terminal expression construct | ||
| chrS-kin-ab-F2 | CTACCTCTGAACATATGGCAGGCCGG | |
| chrS-kin-R | GTCGACGGATCATGAAGTCTCCCG | |
| dtxR cloning | ||
| DTRB | AAAGACATAGGATCCTATTAAAAGCAATC | |
| DTRS | ATCTAATTTGTCGACTTTAGTATTTAGAG |
a
Restriction sites are underlined.