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. 2010 Jul 2;192(18):4776–4785. doi: 10.1128/JB.00261-10

FIG. 3.

FIG. 3.

Generation of an MSMEG_0220 disrupted mutant of M. smegmatis. (A) Schematic diagram of the MSMEG_0220 disrupted gene construct. The black boxes and those with horizontal dashes depict the gene sequence of MSMEG_0220 and the flanking regions used to obtain the disrupted construct, respectively. The part of the MSMEG_0220 gene replaced by the Km gene cassette (gray box) is shown as a hatched box. The primer pairs P3/P6, P5/P4, and P5/P6 were used to confirm the MSMEG_0220 gene disruption. The predicted sizes of the PCR products are indicated in each case. (B) PCR analysis of the wild-type mc2155 (lanes 1, 3, and 5) and recombinant MsΔ0220 (lanes 2, 4, and 6) strains, carried out using various combinations of specific primers (see Table S1 in the supplemental material). M, 1-kb DNA ladder (Promega).