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. 2010 Jul 2;192(18):4776–4785. doi: 10.1128/JB.00261-10

FIG. 5.

FIG. 5.

Growth characteristics of M. smegmatis wild-type (A and E), MsΔ0220 (B and F), ComMsΔ0220 (C and G), and ComMsΔ0220S111A (D and H) strains. (A to D) Five-day-old colonies of M. smegmatis strains on 7H11 solid medium, viewed from the top of the colony, showing rough (A, C, and D) and smooth (B) morphologies. (E to H) Optical microscopy (magnification, ×63) observation of M. smegmatis strains grown in Tween 80-free 7H9 broth. M. smegmatis wild-type (E), ComMsΔ0220 (G), and ComMsΔ0220S111A (H) strains developed cell aggregates, while MsΔ0220 bacteria (F) showed a homogeneous cell suspension with no aggregation.