TABLE 3.
Vectors and plasmids
| Plasmid | Genotype | Primer pair(s) used for cloning | Reference or source |
|---|---|---|---|
| pAC6 | bla lacZ cat amyE′…′amyE | 54 | |
| pGEMcat | ori(f1) lacZ′(MCS) bla cat | 63 | |
| pET-16b | T7 lac promoter, His10 tag, MCS, T7 terminator; lacI pBR322-Ori bla | Novagen | |
| pMAD | erm ori(pE194-Ts) MCS-PclpB-bgaB ori(pBR322) bla | 2 | |
| pAJu604 | pAC6 PydhE(−74)-lacZ | TM1395/TM0480 | This study |
| pDW103 | pMAD PliaI-liaIH up/down overlap | TM1056/TM1057, TM1055/TM1058 | This study |
| pDW105 | pMAD liaR-terminator-gerAC overlap | TM1184/TM1185, TM1186/TM1187 | This study |
| pDW602 | pAC6 PydhE(−96)-lacZ | TM1559/TM0480 | This study |
| pDW604 | pAC6 PydhE(−71)-lacZ | TM1583/TM0480 | This study |
| pDW1604 | pET16b-liaR | TM1045/TM0124 | This study |
| pFK1601 | pET16b-liaH | TM0118/TM0120 | This study |
| pJR601 | pAC6 PydhE(-57)-lacZ | TM0682/TM0480 | This study |
| pJR602 | pAC6 PydhE(-34)-lacZ | TM0683/TM0480 | This study |
| pSJ102 | pMAD liaF up/down overlap | TM0457/TM0574, TM0575/TM0458 | This study |
| pSK602 | pAC6 PydhE-lacZ | TM0480/TM0481 | This study |