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Cellular location of AmiA-GFP, NlpA-GFP, and modified forms of these proteins lacking the TatA signal peptide (AmiAnoSP-GFP) and the lipobox (NlpAnoLB-GFP). E. coli DH5α cells expressing the constructs were fractionated into periplasmic (P), membrane (M), and cytoplasmic (C) fractions, and Western blots were performed with anti-GFP antibody.
