TABLE 1.
Diffusion coefficients determined for GFP constructs in the cytoplasm of E. coli cells (unless indicated otherwise)a
| Proteinb | Molecular mass (kDa) | D (μm2 s−1) | Treatment | Source or reference |
|---|---|---|---|---|
| GFP in water | 27 | 87 | 26 | |
| GFP | 27 | 7.7 ± 2.5 | Induced with 100 μM IPTGc | 6 |
| GFP | 27 | 3.6 ± 0.7 | Induced with 1 mM IPTG | 6 |
| EYFP | 26.5 | 7.08 ± 0.3 | 12 | |
| GFP-His6 | 27+ | 4.0 ± 2.0 | 6 | |
| cMBP-GFP | 72 | 2.5 ± 0.6 | 6 | |
| CheY-GFP | 41 | 4.6 ± 0.8 | 3 | |
| CFP-CheW-YFP | 71 | 1.5 ± 0.05 | 12 | |
| CFP-CheR-YFP | 86.2 | 1.7 ± 0.05 | 12 | |
| torA-GFP | 30 | 9.0 ± 2.1 | Cephalexin | 18 |
| GFP | 27 | 9.8 ± 3.6 | Cephalexin | 28 |
| GFP | 27 | 0.4 ± 0.3 | After osmotic upshock with NaCl | 28 |
| GFP | 27 | 6.3 ± 1.1 | 25 | |
| GFP | 27 | 3.1 ± 1.0 | After osmotic shock | 25 |
| torA-GFP2 in ΔtatABCDE strain | 57 | 7.5 ± 3.9 | Cephalexin, 2% arabinose | This study |
| GFP2 | 27 | 9.1 ± 5.1 | Cephalexin | This study |
| torA-GFP2 | 57 | 8.3 ± 4.2 | Cephalexin, 500 μM arabinose | This study |
| torA-GFP3 | 84 | 6.3 ± 2.6 | Cephalexin, 200 μM arabinose | This study |
| torA-GFP4 | 111 | 5.5 ± 1.9 | Cephalexin, 1 mM arabinose | This study |
| torA-GFP5 | 138 | 2.8 ± 1.5 | Cephalexin, 800 μM arabinose | This study |
| AmiA-GFP | 58 | 1.8 ± 0.8 | 2% arabinose | This study |
| AmiA-GFP | 58 | 1.8 ± 1.2 | Cephalexin, 2% arabinose | This study |
| AmiAnoSP-GFP | 58 | 7.1 ± 3.6 | Cephalexin, 2% arabinose | This study |
| NlpA-GFP | 55 | 2.1 ± 1.4 | Cephalexin, 2% arabinose | This study |
| NlpAnoLB-GFP | 55 | 2.7 ± 3.2 | Cephalexin, 2% arabinose | This study |
a
The techniques used were FRAP and photoactivation of a red-emitting fluorescence state of GFP (6), fluorescence correlation spectroscopy (3), confocal FRAP (12, 18), pulsed FRAP (28), and continuous photobleaching with evanescent illumination (25).
b
EYFP, enhanced yellow fluorescent protein; cMBP, cytoplasmic maltose-binding protein.
c
IPTG, isopropyl-β-D-thiogalactopyranoside.