FIG. 2.

Deletion analysis of the 14.1-kb HindIII fragment containing bphS2T2. (A) Physical maps of deletion derivatives. The segments represented by solid bars were inserted into pKLAF1, which is a reporter plasmid that does not contain any bphST segment. (B) Luciferase assay of the deletion derivatives. The IAM1399 cells harboring the plasmids were grown in 1/5 LB in the presence or absence of ethylbenzene and were subjected to the luciferase assay. The activity was expressed as RLU measured by a luminometer per milliliter of culture per OD₆₀₀ unit, as described in Materials and Methods. The data are means ± standard deviations from at least three independent experiments.