FIG. 6.

Cross-communication between heterologous BphS and BphT. An original bphT was replaced by a heterologous bphT at the common ApaI site to obtain pKLAS1T2 and pKLAS2T1 containing bphS1 plus bphT2 and bphS2 plus bphT1, respectively. Shown is the luciferase activity of IAM1399 harboring pKLAS1T2 or pKLAS2T1. The cells were incubated in 1/5 LB in the presence or absence of biphenyl or ethylbenzene for 5 h and subjected to a luciferase assay. IAM1399 harboring pKLAST1 or pKLAST2 was used as a control. The activity was expressed as in Fig. 2. The data are means ± standard deviations from at least three independent experiments.