TABLE 2.
Induction of BphS1T1-governing promoters by BphS2T2
| Promoter | Plasmid | bphS2T2b | Luciferase activitya (105) |
||
|---|---|---|---|---|---|
| None | BPH | ETB | |||
| bphAap | pKLAF1 | − | 3 ± 0.1 | 3 ± 0.1 | 3 ± 0.2 |
| pKMBPH | + | 39 ± 1 | 36 ± 2 | 381 ± 9 | |
| etbAa1p | pKLABD1 | − | 3 ± 0.2 | 3 ± 0.01 | 2 ± 0.2 |
| pKMETBA | + | 39 ± 2 | 36 ± 1 | 419 ± 9 | |
| etbAa2p | pKLAED2 | − | 3 ± 0.1 | 2 ± 0.2 | 2 ± 0.1 |
| pKMEBDA | + | 22 ± 1 | 19 ± 0.4 | 426 ± 10 | |
| etbAdp | pKLAEA4 | − | 2 ± 0.2 | 3 ± 0.2 | 3 ± 0.2 |
| pKMETA4 | + | 13 ± 2 | 19 ± 0.2 | 304 ± 12 | |
| etbD1p | pKLAED1 | − | 1 ± 0.2 | 1 ± 0.2 | 1 ± 0.2 |
| pKMETBD | + | 9 ± 0.1 | 11 ± 0.7 | 271 ± 8 | |
a
The plasmids were separately introduced into IAM1399, and the resultant transformants were grown in 1/5 LB in the presence or absence (None) of biphenyl (BPH) or ethylbenzene (ETB), and were subjected to a luciferase assay. The activity was expressed as in Fig. 2. The data are means ± standard deviations from at least three independent experiments.
b
The presence (+) or absence (−) of bphS2T2 is indicated.