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. 2010 Jul 16;192(18):4562–4570. doi: 10.1128/JB.00536-10

FIG. 1.

FIG. 1.

Southern blotting reveals the presence of two copies of dosR in recombinant W/Beijing strains. Genomic DNA from wild-type and two independent dosR::hyg recombinants generated for H37Rv (clones 19 and 24) and the W/Beijing isolates HN878 (clones 25 and 28) and G4B1.2 (clones 30 and 32) was digested with PstI and transferred by Southern blotting. (A) The membrane was hybridized with a 2.4-kb PCR fragment (generated using dosR-1 and dosS-1) that includes Rv3134c, dosR, and 739 bp of dosS. Note the presence of an intact copy of dosR retained by the HN878 and G4B1.2 dosR::hyg strains. (B) Based on the published H37Rv sequence, a genetic map of the dosR region in the recombinant strains is shown. By replacing 486 bp of dosR with the hyg resistance cassette, an additional PstI site has been introduced.