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Localization of pili in wild-type (WT) S. pyogenes and various deletion mutants. Proteins in wall (W) and culture supernatant (S) fractions were separated by SDS-PAGE on a 4 to 15% (wt/vol) gel and immunoblotted with anti-rSpy0128. The arrow at the right indicates unpolymerized Spy0128 monomers. The intensity of the band detected in the Δspy0129 mutant suggests that monomers may be degraded if not incorporated into pili.
