TABLE A1.
Immunogenic proteins of Streptococcus pyogenes identified by the Antigenome technology
| ORF | Common name | No. of clonesa | No. of screensb | Library:no. of clones per ORFc | Location of immunogenic region (aa)d |
|---|---|---|---|---|---|
| Spy0012 | Hypothetical protein | 19 | 3 | A:12, I:5, N:2 | 1-114 |
| Spy0019 | Putative secreted protein (cell division and antibiotic tolerance) | 83 | 5 | F:2, I:16, K:24, N:29, P:12 | 29-226 |
| Spy0025 | Putative phosphor-ribosylformylglycinamidine synthase II | 3 | 1 | D:3 | 919-929 |
| Spy0031 | Putative choline-binding protein | 9 | 3 | I:3, K:3, N:3 | 145-305 |
| Spy0103 | Putative competence protein | 8 | 1 | A:8 | 71-81 |
| Spy0112 | Putative pyrroline carboxylate reductase | 4 | 1 | B:4 | 173-186 |
| Spy0115 | Putative glutamyl-aminopeptidase | 29 | 2 | A:3, C:26 | 316-331 |
| Spy0166 | Hypothetical protein | 82 | 5 | I:22, K:7, N:17, O:31, P:5 | 21-99 |
| Spy0167 | Streptolysin O | 1,465 | 14 | A:118, B:14, C:18, D:37, F:141, G:79, H:92, I:97, K:123, L:5, M:21, N:225, O:230, P:265 | 9-264 |
| Spy0168 | Hypothetical protein | 11 | 2 | K:4, N:7 | 1-112 |
| Spy0171 | Hypothetical protein | 2 | 1 | H:2 | 21-56 |
| Spy0183 | Putative glycine betaine/proline ABC transporter | 6 | 1 | C:6 | 23-39 |
| Spy0230 | Putative ABC transporter (ATP-binding protein) | 46 | 1 | C:46 | 474-489 |
| Spy0269 | Putative surface exclusion protein | 39 | 6 | A:2, B:12, D:3, F:11, H:5, N:6 | 37-241 |
| 409-534 | |||||
| 582-604 | |||||
| 743-804 | |||||
| Spy0287 | Conserved hypothetical protein | 1 | 1 | K:1 | 202-337 |
| Spy0292 | Penicillin-binding protein | 2 | 1 | F:2 | 1-48 |
| Spy0295 | Oligopeptide permease | 3 | 1 | A:3 | 203-217 |
| Spy0348 | Putative aminodeoxychorismate lyase | 14 | 4 | D:5, I:3, M:3, P:3 | 261-273 |
| Spy0416 | Putative cell envelope serine proteinase | 462 | 13 | A:3, B:4, C:30, D:13, F:134, G:120, H:93, I:9, K:13, M:2, N: 14, O:8, P:19 | 1-414 |
| 443-614 | |||||
| 997-1392 | |||||
| Spy0430 | Hypothetical protein | 35 | 3 | B:7, I:10, P:18 | 1-164 |
| Spy0433 | Hypothetical protein | 295 | 9 | A:138, B:8, C:67, D:11, E:13, F:35, G:10, H:5, M:8 | 126-207 |
| Spy0437 | Hypothetical protein | 99 | 5 | A:29, B:10, C:21, D:24, E:15 | 180-204 |
| Spy0469 | Putative 42-kDa protein | 145 | 7 | B:5, F:77, I:8, K:15, M:3, N:17, O:20 | 11-197 |
| 204-219 | |||||
| 258-372 | |||||
| Spy0488 | Hypothetical protein | 72 | 7 | A:17, B:11, C:23, D:11, G:4, H:6 | 195-289 |
| Spy0515 | Putative sugar transferase | 8 | 2 | B:5, I:3 | 12-130 |
| Spy0580 | Conserved hypothetical protein | 5 | 1 | C:5 | 434-444 |
| Spy0621 | Conserved hypothetical protein | 3 | 1 | C:3 | 360-375 |
| Spy0630 | Putative PTSe-dependent N-acetyl-galactosamine IIC | 2 | 1 | C:2 | 254-260 |
| Spy0681 | Hypothetical protein, phage associated | 8 | 1 | A:8 | 369-382 |
| Spy0683 | Putative minor capsid protein, phage associated | 15 | 2 | B:11, D:4 | 270-312 |
| Spy0702 | Hypothetical protein | 2 | 1 | L:2 | 486-598 |
| Spy0710 | Conserved hypothetical protein, phage associated | 10 | 1 | B:10 | 378-396 |
| Spy0711 | Pyrogenic exotoxin C precursor, phage associated (speC) | 2 | 1 | K:2 | 75-235 |
| Spy0720 | Conserved hypothetical protein | 2 | 1 | D:2 | 30-51 |
| Spy0727 | Putative DNA gyrase, subunit B | 26 | 1 | M:26 | 208-219 |
| Spy0737 | Putative extracellular matrix-binding protein | 19 | 3 | B:5, E:3, K:11 | 396-533 |
| 1342-1502 | |||||
| 1672-1920 | |||||
| Spy0747 | Extracellular nuclease | 98 | 4 | A:72, B:17, H:6, O:3 | 1-113 |
| 210-232 | |||||
| 250-423 | |||||
| 536-564 | |||||
| Spy0777 | Putative ATP-dependent exonuclease, subunit A | 6 | 2 | C:4, E:2 | 617-635 |
| Spy0789 | Putative ABC-transporter (permease protein) | 3 | 1 | A:3 | 190-203 |
| Spy0839 | Putative glycerophosphodiester phosphodieste | 9 | 2 | A:7, D:2 | 385-398 |
| Spy0843 | Cell surface protein | 1,079 | 14 | A:11, B:3, C:5, D:4, F:50, H:19, G:49, I:112, K:102, L:10, M:3, N:213, O:188, P:310 | 12-190 |
| 276-283 | |||||
| 666-806 | |||||
| Spy0872 | Putative secreted 5′-nucleotidase | 75 | 9 | A:6, D:2, F:5, H:14, I:9, K:10, L:1, N:16, O:12 | 30-80 |
| 89-105 | |||||
| 111-151 | |||||
| Spy0895 | Histidine protein kinase | 11 | 1 | C:11 | 195-203 |
| Spy0972 | Putative terminase, large subunit, phage | 2 | 1 | B:2 | 32-50 |
| Spy0981 | Hypothetical protein, phage associated | 9 | 2 | A:7, B:2 | 75-90 |
| Spy1008 | Streptococcal exotoxin H precursor (speH) | 11 | 1 | C:11 | 69-88 |
| Spy1032 | Extracellular hyaluronate lyase | 11 | 3 | B:3, K:3, M:5 | 96-230 |
| 361-491 | |||||
| 572-585 | |||||
| Spy1054 | Putative collagen-like protein (SclC) | 1,295 | 9 | A:71, B:13, C:233, D:41, E:163, F:200, G:442, H:129, N:3 | 102-210 |
| Spy1063 | Putative periplasmic-iron-binding protein | 4 | 1 | A:4 | 240-248 |
| Spy1162 | Putative RNase HII | 8 | 2 | B:3, C:5 | 182-198 |
| Spy1206 | Putative ABC transporter | 2 | 1 | A:2 | 41-56 |
| Spy1228 | Putative lipoprotein | 33 | 1 | M:33 | 202-217 |
| Spy1245 | Putative phosphate ABC transporter | 6 | 2 | I:3, K:3 | 1-127 |
| Spy1315 | Hypothetical protein | 4 | 1 | B:4 | 297-458 |
| Spy1357 | Protein GRAB (protein G-related alpha 2 M-binding protein) | 41 | 4 | G:27, H:8, K:2, N:4 | 24-135 |
| Spy1361 | Putative internalin A precursor | 62 | 5 | F:21, G:26, H:6, K:4, N:5 | 176-330 |
| Spy1371 | Putative NADP-dependent glyceraldehyde-3-phosphate dehydrogenase | 17 | 2 | D:14, H:3 | 46-62 |
| 296-341 | |||||
| Spy1375 | Putative ribonucleotide reductase alpha-c | 2 | 1 | A:2 | 667-684 |
| Spy1389 | Putative alanyl-tRNA synthetase | 5 | 2 | B:2, P:3 | 258-416 |
| Spy1390 | Putative protease maturation protein | 8 | 3 | A:3, B:2, D:3 | 278-295 |
| Spy1422 | Putative recombination protein | 2 | 1 | C:2 | 183-195 |
| Spy1436 | Putative DNase | 1 | 1 | K:1 | 63-238 |
| Spy1494 | Hypothetical protein | 39 | 7 | G:3, I:5, K:6, M:5, N:10, O:6, P:4 | 1-141 |
| Spy1523 | Cell division protein | 2 | 1 | I:2 | 231-368 |
| Spy1536 | Conserved hypothetical protein | 22 | 2 | A:19, C:3 | 247-260 |
| Spy1564 | Conserved hypothetical protein | 4 | 1 | C:4 | 64-72 |
| Spy1604 | Conserved hypothetical protein | 4 | 2 | B:2, K:2 | 222-362 |
| 756-896 | |||||
| Spy1607 | Conserved hypothetical protein | 5 | 1 | D:5 | 153-170 |
| Spy1615 | Putative late competence protein | 4 | 1 | C:4 | 56-73 |
| Spy1666 | Conserved hypothetical protein | 2 | 1 | D:2 | 298-312 |
| Spy1727 | Conserved hypothetical protein | 6 | 1 | B:5 | 141-157 |
| Spy1785 | Putative ATP-dependent DNA helicase | 3 | 1 | D:3 | 433-440 |
| 572-593 | |||||
| Spy1798 | Hypothetical protein | 69 | 6 | A:12, I:12, K:7, N:17, O:13, P:8 | 17-319 |
| 417-563 | |||||
| Spy1801 | Immunogenic secreted protein precursor homolog | 27 | 4 | H:2, I:8, K:6, N:11 | 46-187 |
| Spy1813 | Hypothetical protein | 34 | 3 | I:16, K:12, N:6 | 21-244 |
| 381-499 | |||||
| 818-959 | |||||
| Spy1821 | Putative translation elongation factor EF-P | 6 | 1 | C:6 | 118-136 |
| Spy1916 | Putative phospho-beta-d-galactosidase | 8 | 1 | C:8 | 147-155 |
| Spy1972 | Pullulanase | 22 | 4 | A:6, I:2, K:5, N:9 | 74-438 |
| Spy1979 | Streptokinase A precursor | 19 | 3 | I:6, M:3, N:10 | 156-420 |
| Spy1983 | Collagen-like surface protein (SclD) | 169 | 6 | A:81, B:24, F:19, G:41, I:2, K:2 | 79-348 |
| Spy1991 | Anthranilate synthase component II | 2 | 1 | D:2 | 53-70 |
| Spy2000 | Surface lipoprotein | 5 | 2 | B:3, N:2 | 183-341 |
| Spy2006 | Hypothetical protein | 95 | 9 | A:15, B:9, C:5, D:3, F:18, G:25, H:5, M:10, N:5 | 92-231 |
| 618-757 | |||||
| Spy2009 | Hypothetical protein | 18 | 4 | B:2, I:7, K:7, P:2 | 41-170 |
| Spy2010 | C5A peptidase precursor | 172 | 11 | A:47, B:10, D:3, F:48, G:20, H:4, I:6, K:13, M:5, N:10, P:6 | 20-487 |
| 757-1153 | |||||
| Spy2016 | Inhibitor of complement (Sic) | 185 | 10 | A:11, B:38, C:16, F:56, G:27, H:13, K:5, N:2, O:3, P:14 | 26-74 |
| 91-100 | |||||
| 105-303 | |||||
| Spy2018 | M1 protein | 783 | 12 | A:316, B:26, C:107, D:12, E:49, F:88, G:118, H:6, I:7, K:2, M:48, N:4 | 10-223 |
| 231-251 | |||||
| 264-297 | |||||
| 312-336 | |||||
| Spy2025 | Immunogenic secreted protein precursor | 155 | 7 | F:7, G:16, H:7, K:63, L:2, N:18, O:42 | 22-344 |
| Spy2039 | Pyrogenic exotoxin B | 30 | 3 | I:15, K:3, N:12 | 1-151 |
| Spy2043 | Mitogenic factor MF1 (speF) | 1 | 1 | K:1 | 91-263 |
| Spy2059 | Penicillin-binding protein 2a | 4 | 2 | D:2, E:2 | 261-272 |
| Spy2110 | Putative anaerobic ribonucleoside-triphosphate reductase | 7 | 1 | E:7 | 541-551 |
| Spy2127 | Hypothetical protein | 8 | 2 | I:6, P:2 | 84-254 |
| Spy2191 | Hypothetical protein | 28 | 3 | C:20, E:3, M:5 | 61-78 |
| Spy2211 | Transmembrane protein | 3 | 1 | A:3 | 568-580 |
a
Number of hits for a particular antigen.
b
Number of screens in which the antigen was selected.
c
A, LSPy-70 library in lamB with IC3-IgG (number of clones successfully sequenced, 1,588); B, LSPy-70 library in lamB with IC3-IgA (1,539); C, LSPy-70 library in lamB with IC6-IgG (1,173); D, LSPy-70 library in lamB with P4-IgG (1,138); E, LSPy-70 library in lamB with P4-IgA (981); F, LSPy-150 library in btuB with IC3-IgG (991); G, LSPy-150 library in btuB with IC6-IgG (1,036); H, LSPy-150 library in btuB with P4-IgG (681); I, LSPy-400 library in fhuA with IC3-IgG (559); K, LSPy-400 library in fhuA with IC6-IgG (543); L, LSPy-400 library in fhuA with P4-IgG (20); M, LSPy-70 library in lamB with P13-IgG (796); N, LSPy-400 library in fhuA with P13-IgG (762); O, LSPy-400 library in fhuA with P13-IgA (652); P, LSPy-400 library in fhuA with IC3-IgA (741).
d
Amino acid (aa) positions of the ORF borne by selected clones.
e
PTS, phosphotransferase system.