FIG. 2.

Two-dimensional Coomassie-stained gel of proteins in the pooled culture supernatants of C. perfringens type C isolates. Proteins were separated in the first dimension by a pH 4 to 7 immobilized pH gradient gel (length, 17 cm) and then in the second dimension by a 12% polyacrylamide gel. Spots were excised, and the corresponding proteins were identified by MALDI-TOF-MS and database searches. The spots are labeled on the gel according to the numbers presented in Table 1 and Table S2 in the supplemental material.