TABLE 1.
Extracellular proteins identified from C. perfringens ATCC 13124 and pooled exoproteomes of type C strains
| Spot no. | Protein name (accession no.) |
Mr/pI |
COG (functional category)b | Pfam match | |
|---|---|---|---|---|---|
| Theoreticala | Observed | ||||
| A2 (IM1)c | Endo-beta-N-acetylglucosaminidase (YP_695266) | 126/4.66 | 120/4.8 | No related COG | Uncharacterized protein conserved in bacteria |
| A3 (IM6)c | Acetyl-CoA acetyltransferase (YP_696874) | 40/6.06 | 43/6.6 | Acetyl-CoA acetyltransferases, COG0183 (I) | Thiolase, N-terminal domain |
| A4 | Polysaccharide deacetylase family protein (ABG83723) | 34/6.93 | 37/6.7 | Predicted xylanase/chitin deacetylase, COG0726 (G) | Polysaccharide deacetylase |
| A5 | Putative lipoprotein (YP_696216) | 51/6.08 | 48/6.5 | No related COG | No Pfam match |
| A6 | Hypothetical protein CPF_1441 (YP_695886) | 25/5.21 | 28/5.6 | NifU homologs involved in Fe-S cluster formation, COG0822 (C) | NifU-like N terminal domain |
| A7 | Putative rubrerythrin (YP_697309) | 19/5.43 | 21/5.42 | Rubrerythrin, COG1592 (C) | Rubrerythrin |
| A8 | Triosephosphate isomerase (YP_695952) | 27/5.01 | 27/5.3 | Triosephosphate isomerase, COG0149 (C) | Triosephosphate isomerase |
| A9 | Fructose-bisphosphate aldolase (YP_696000) | 30/5.02 | 29/5.4 | Fructose bis-phosphate aldolase, COG0191 (G) | Fructose-bisphosphate aldolase class II |
| A10 | Hypothetical protein CPF_2918 (YP_697282) | 31/4.88 | 43/4.9 | No related COG | Protein of unknown function |
| A11 | SagA protein (YP_694734) | 46/5.61 | 43/4.2 | Cell wall-associated hydrolases (invasion-associated proteins), COG0791 (M) | NlpC/P60 family |
| A12 | Probable ion uptake ABC transporter (YP_694897) | 38/5.20 | 37/5.08 | ABC-type iron/thiamine transport systems, COG1840 (I) | Bacterial extracellular solute-binding protein |
| A13 (IM16)c | Fructose-bisphosphate aldolase (YP_696000) | 30/5.02 | 49/4.01 | Fructose bis-phosphate aldolase, COG0191 (G) | Fructose-bisphosphate aldolase class II |
| A14 (IM18)c | Extracellular solute-binding protein (YP_696758) | 54/4.7 | 47/4.79 | Sugar-binding periplasmic proteins/domains COG1653 (G) | Bacterial extracellular solute-binding protein |
| A15 | DnaK-type molecular chaperone hsp70 (YP_696713) | 66/4.75 | 80/4.9 | Molecular chaperone, COG0443 (O) | Hsp70 protein |
| A16 (IM13)c | SagA protein (YP_694734) | 46/5.61 | 48/5.75 | Cell wall-associated hydrolases (invasion-associated proteins), COG0791 (M) | NlpC/P60 family |
| A17 (IM12)c | Phospholipase C (YP_694509) | 42/5.24 | 45/5.6 | No related COG | No Pfam match |
| A18 | SagA protein (YP_694734) | 46/5.61 | 48/5.8 | Cell wall-associated hydrolases (invasion-associated proteins), COG0791 (M) | NlpC/P60 family |
| A21 | M24/M37 family peptidase (YP_694655) | 44/5.67 | 48/6.0 | Membrane proteins/ metalloendopeptidase, COG0739 (M) | Peptidase family M23 |
| A22 (IM9)c | Ornithine carbamoyltransferase (YP_694626) | 37/5.4 | 43/6.1 | Ornithine carbamoyltransferase, COG0078 (E) | Aspartate/ornithine carbamoyltransferase |
| A23 | Glyceraldehyde-3-phosphate dehydrogenase (YP_695954) | 35/5.5 | 38/6.15 | Glyceraldehyde-3-phosphate dehydrogenase, COG0057 (G) | Glyceraldehyde 3-phosphate dehydrogenase |
| A24 | Hypothetical protein CPF_0656 (YP_695109) | 37/5.53 | 35/5.49 | No related COG | No Pfam match |
| A26 | F5/8 type C domain-containing protein (YP_695932) | 189/5.15 | 110/5.5 | No related COG | No Pfam match |
| A27 | Hypothetical protein CPF_2364 (YP_696787) | 123/5.18 | 105/5.5 | No related COG | No Pfam match |
| A29 | Purine-nucleoside phosphorylase (YP_697182) | 29/5.63 | 29/6.1 | Purine nucleoside phosphorylase, COG0005 (F) | Phosphorylase superfamily |
| A30 | Hypothetical protein CPF_0656 (YP_695109) | 37/5.53 | 35/5.34 | No related COG | No Pfam match |
| A31 | Cell wall binding repeat-containing protein (YP_695059) | 63/5.37 | 50/5.82 | Predicted carboxypeptidase, COG2866 (E) | Putative cell wall binding repeat |
| A32 | Exo-alpha-sialidase (YP_695173) | 77/5.43 | 95/5.7 | No related COG | No Pfam match |
| A33 (IM14)c | Translation elongation factor (YP_696386) | 33/5.13 | 37/5.6 | Translation elongation factor Ts, COG0264 (J) | Elongation factor TS |
| A34 | S-Adenosylmethionine synthetase (YP_696856) | 37/5.17 | 45/5.5 | S-Adenosylmethionine synthetase, COG0192 (H) | S-Adenosylmethionine synthetase, N-terminal domain |
| A35 | Peptidyl-prolyl cis-isomerase family protein (YP_694723) | 27/4.89 | 34/5.2 | Parvulin-like peptidyl-prolyl isomerase, COG0760 (O) | Rotamase |
| A36 | F5/8 type C domain-containing protein (YP_695932) | 189/5.15 | 110/4.5 | No related COG | No Pfam match |
| C1 | Alpha-clostripain (NP_562197) | 59/5.88 | 40/5.5 | No related COG | No Pfam match |
| C2 | Alpha-clostripain trans (NP_562197) | 59/5.88 | 40/5.65 | No related COG | No Pfam match |
| C4 | Alpha-clostripain (NP_562197) | 59/5.88 | 40/4.8 | No related COG | No Pfam match |
| C5 | Glyceraldehyde-3-phosphate dehydrogenase (YP_695954) | 35/5.5 | 38/5.82 | Glyceraldehyde-3-phosphate dehydrogenase, COG0057 (G) | Glyceraldehyde 3-phosphate dehydrogenase |
| C6 | Alpha-clostripain (NP_562197) | 59/5.88 | 21/4.98 | No related COG | No Pfam match |
| C7 | Alpha-clostripain (NP_562197) | 59/5.88 | 20/4.98 | No related COG | No Pfam match |
| C8 | Alpha-clostripain (NP_562197) | 59/5.88 | 18/5.05 | No related COG | No Pfam match |
| C10 | Collagenase A (NP_561089) | 125/4.93 | 110/5.15 | PKD repeat proteins, COG3291 (R) | Peptidase_M9 |
| C11 | Collagenase A (NP_561089) | 125/4.93 | 105/4.5 | PKD repeat proteins, COG3291 (R) | Peptidase_M9 |
| C13 | Acetyl-CoA acetyltransferase (YP_696874) | 40/6.06 | 39/6.7 | Acetyl-CoA acetyltransferases, COG0183 (I) | Thiolase, N-terminal domain |
| C14 | Glyceraldehyde-3-phosphate dehydrogenase (YP_695954) | 35/5.5 | 38/6.21 | Glyceraldehyde-3-phosphate dehydrogenase, COG0057 (G) | Glyceraldehyde 3-phosphate dehydrogenase |
| C15 | Glyceraldehyde-3-phosphate dehydrogenase (YP_695954) | 35/5.5 | 38/6.01 | Glyceraldehyde-3-phosphate dehydrogenase, COG0057 (G) | Glyceraldehyde 3-phosphate dehydrogenase |
| C16 | Hypothetical protein CPF_2364 (YP_696787) | 123/5.18 | 28/5.79 | No related COG | No Pfam match |
| C17 | Probable heme oxygenase (YP_694668) | 25/5.18 | 27/5.70 | No related COG | No Pfam match |
| C18 | HPr family phosphocarrier protein (YP_696356) | 9/6.5 | 15/6.4 | Phosphotransferase system, HPr-related proteins, COG1925 (G) | PTS-HPr |
| C21 | DnaK-type molecular chaperone hsp70 | 66/4.79 | 95/4.95 | Molecular chaperone, COG0443 (O) | Hsp70 protein |
| C23 | HPr family phosphocarrier protein (YP_696356) | 9/6.59 | 15/6.83 | Phosphotransferase system, HPr-related proteins, COG1925 (G) | PTS-HPr |
a
Theoretical values were obtained with an online tool at http://expasy.org/sprot/.
b
COGs were assigned after a COGnitor search. Functional role categories, assigned according to the descriptions on the COG page at http://www.ncbi.nlm.nih.gov/COG, are as follows: E, amino acid transport and metabolism; M, cell envelope biogenesis, outer membrane; G, carbohydrate transport and metabolism; O, posttranslational modification, protein turnover, chaperones; C, energy production and conversion; J, translation, ribosomal structure and biogenesis; H, coenzyme transport and metabolism; I, lipid metabolism; F, nucleotide transport and metabolism; R, general function prediction only.
c
Immunoreactive to serum raised against C. perfringens whole-cell culture in mice.