FIG. 1.

Physical map around the gtfB gene and properties of the gtfB mutant. (A) A physical map around the gtfB gene region. A triangle indicates the Tn4400′ insertion site of strain KDP390. (B) Colonial pigmentation. P. gingivalis cells were anaerobically grown on blood agar plates at 37°C. (C) Hemagglutination. P. gingivalis cells were grown in enriched BHI broth, washed with PBS, and suspended in PBS at an optical density at 540 nm of 0.3. The suspension and its dilutions in a 2-fold series were applied to the wells of a microtiter plate from left to right and mixed with sheep erythrocyte suspension.